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Multi-link Multi-stage Stainless Steel Bioreactor

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Multi-link Multi-stage Stainless Steel Bioreactor


10L50L3 grade 2 stainless steel fermenter


10L50L3-set 2-grade stainless steel fermentation tank BLBIO-10SJ-3-50SJ


15L50L150L500L multi-stage fermenter


Multiple fermenters (with or without different volumes) can be used to form a conjoined system, which is suitable for parallel orthogonal tests, providing an optimal approach to process research and shortening valuable research time. The key is that the process parameters of this equipment configuration are relatively repeatable.


BLBIO-C type control system: German Siemens S7-200 series PLC control system +10" LCD touch screen display operation, on-site direct operation (menu type), full Chinese menu and interface; Sampling and display of tank data, browsing and analysis of system data, reading settings. It can control the stirring speed, the temperature of the medium in the tank, the pH value, and the DO value;

It can set the upper and lower limits of temperature, speed, PH value, DO value, and has the function of over-limit alarm;

It has the functions of over-level alarm and automatic refill, over-level alarm and automatic addition of defoamer;

All alarm accidents can be recorded and consulted;

Speed, temperature, PH value, DO value, etc. can be set to automatic and off state;

Parameters such as medium temperature, stirring speed, Ph, DO and other parameters in the tank can be set and controlled in sections according to the process requirements;

Each parameter has PID adjustment process display, such as set value, real-time value, PID set value, upper and lower limit value, curve display, manual/automatic switching, online setting, etc.;

The historical data and curves of various parameters can be stored for many years, and the real-time curves can be restored as they are when the power is turned on again;

The man-machine interface is friendly, and more than a dozen monitoring screens can be switched;

It has the function of feeding and metering; Regular and quantitative feeding;

The system has a variety of correlation controls: correlation control between velocity and dissolved oxygen, correlation control between feeding and dissolved oxygen, correlation control between feeding and pH value, etc.;

The system has real-time display, data recording and data analysis of the running process

The parameters of each detection and control loop can be manually set and corrected online on the screen, and the temperature, DO, PH sensors can be set and adjusted, and the flow rate of each peristaltic pump can be calibrated. The operation has a password protection function, and the password is divided into operator and administrator-level passwords;

Automatically record the fermenter start-up time, shutdown time, number of power outages during automatic operation, power outage time, call time, etc.;

The software system has self-adaptive and self-diagnostic capabilities;



Parameter

Description

Total volume

5L,10L,15L,20L,30L,50L,100L,200L,500L,1000L, 5KL, 50KL, 200KL etc.

Working volume

30%~70%

Tank

Jacket tank, made by SS316 and SS304, tank design pressure 0.3Mpa, jacket design pressure 0.35Mpa, polishing 0.4-0.6um, ratio of diameter and height is 1:2.2-2.5

Stirring system

Mechanical drive on the top/bottom or Magnetic drive on the top, 3pcs height adjustable six-blade paddle

Stir speed: 50~1000rpm/50-400rpm

Sterilization

Sterilize in place(SIP), auto control by program

Clean

Clean in place (CIP) by spray ball

Gas control

Control by rotor meter, air flow 1 vvm

Feeding

Automatically feeding by peristaltic pump

Anti-foam control

Automatically add antifoam by peristaltic pump

Temperature control

Auto-control,  electric heating range: water source add 5℃ to 70℃

PH control

PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland.

Do control

0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen.

Sampling

Sampling valve, can sterilization separately, manual

Harvest

Zero dead zone bottom valve(Gemu/Burket,Germany), can sterilization separately, manual/auto

Lid lift system

30L-500L have lid lift system, 700-1000L have human hole.

Pressure

Auto control, BD Germany pressure transmitter

Exhaust

Have condenser before exhaust, control by diagram valve / Proportional control valve.

Control parameters

10 or 15 inch touch screen , Siemens PLC with control parameters (stirring, pH, DO, temperature, feeding, alarm, gas etc.), data analysis, data record, data output, language(RU/EN/CN) and other customized functions

Optional configuration

Remote control, Mass flow meter(auto control gas), O2/N2/CO2, gas flow 2vvm, gas(CO2) exhaust analysis, Level sensor, Turbidity(OD) sensor, ORP sensor, Weight system, Live cell sensor(Biomass), Printing, Siemens touch screen, Double mechanical seal, 4-Valves-group for feeding, Bottom valve auto discharge, ASME certificate, CSA certificate, Batch culture, Seed transfer, etc.


Fermenter Description:

In the multi-stage fermentation tank system, each tank can be used independently or in combination; reliable transplantation pipeline, which can be sterilized separately; floor-standing/bench-mounted racks are optional, separate control cabinets or centralized control; can be based on It is necessary to carry out a combination of various fermenters, which is suitable for the transitional fermentation test research from laboratory shake flask enlargement to production, and can also be used for small-scale production.

The main material is all stainless steel 316L tank body, there is no dead angle in the tank; it is equipped with special sampling and discharging valves for fermentation tanks, and transplanting pipes. It adopts a large viewing angle tank liquid level observation glass, 12V safety light, with temperature, PH, DO, defoaming, liquid level interface, inoculation port, feeding port, and multiple spare ports. All welds are strong, neat and beautiful. The tank body is designed with optimized diversion, which improves the exchange efficiency and the uniformity of the temperature in the tank.One way of deep ventilation is adopted, and the rotameter shows that the gas flow is adjusted according to the process requirements, and the air distributor in the tank distributes the gas evenly; the sterilizing filter (Dominic or Shanghai Yiming) with a precision of 0.2 μm is used to prevent material backflow device, ensuring safety and greatly extending service life.

German Siemens S7-200 series PLC control system + 10” LCD touch screen display operation, on-site direct operation (menu type), full English menu and interface; sampling and display of tank data, browsing and analysis of system data, and reading settings. Available Control the stirring speed, the temperature of the medium in the tank, Ph value, DO value.

Fermenter Application:

Animal vaccine production: Avian influenza vaccine, Porcine PRRS vaccine, Foot-and-mouth disease, Rabies vaccine, Mink vaccine, DTP vaccine, Diphtheria vaccine, Tetanus vaccine, etc.

Human vaccine production: Diphtheria vaccine, Tetanus vaccine, Meningitis vaccine, Pneumonia IV vaccine, Hepatitis B vaccine, etc.

Culture for microorganism (bacterial,fungi,yeast.etc), mammalian cell(293,CHO etc.), plant cell, algae, enzyme, protein, additives, antibiotics etc.


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