BLBIO-WAVE-10/25L Wave Bioreactor Configuration and Technical Parameters

BLBIO-WAVE-10/25L wave bioreactor configuration and technical parameters

First, the main configuration

1. System composition:

The equipment includes the following configurations: swinging bed, tray, heating plate, weighing system, peristaltic pump unit, gas mixing system, Siemens PLC control system;

2 Bedriding: used with trays and disposable culture bags;

Through the control of swing speed, angle, mixed gas and flow, temperature, pH, DO and other parameters, the culture can be effectively mixed and the shear force is reduced, and the load cell, temperature sensor, pH sensor, DO sensor, gas concentration sensor are integrated, and real-time data recording is provided;

2.1 Load cell: It can accurately and continuously measure the weight of the culture, and is associated with the peristaltic pump to achieve continuous perfusion culture;

2.2 Temperature sensor: It can accurately and continuously monitor the temperature of the culture, so that the system can adjust it in time to ensure the stability of the culture temperature;

2.3 pH sensor: can accurately and continuously monitor the pH of the culture, and can be associated with the peristaltic pump to automatically control the alkali replenishment or associated with the gas mixing system to increase the_CO2 gas intake and adjust the pH value;

2.4 DO sensor: it can accurately and continuously monitor the DO of cultures, and can be associated with the gas mixing system to adjust the DO value in time;

2.5 Gas concentration sensor: It can accurately monitor the concentration of_O2 and_CO2 in the culture bag in real time, so that the system can adjust the reasonable gas environment in time;

2.6 Data recording: used to save the running data of the cell culture process, which can be exported to the external computer through the U disk to analyze and mine the data;

3 trays: installed on the bed, easy to install, fix and replace the culture bag, equipped with the corresponding cover;

4. Electric heating plate: It can be heated efficiently and uniformly, and the maximum output power of the heating plate can be limited to achieve accurate, stable and fast temperature control effect, and there are safety protection measures to prevent the risk of overheating;

5. Peristaltic pump set: equipped with 3 pump heads, which are used for inoculation, harvesting, feeding and alkali replenishment. Each peristaltic pump can be automatically controlled in association with weighing;

6. Gas mixing system: used to supply gas to the culture, equipped with a thermal mass flow meter,_O2 and_CO2 concentration sensors, which can monitor and alarm the exhaust gas of the culture bag;

7 control system: using PLC control module, self-developed software, Chinese interface, through the operation of 7-inch color touch screen to monitor and control the status of the operating parameters of the cell culture process, with a high degree of integration; Provides a user-friendly interface to display the real-time status of the operation, including temperature, pH, DO, weight, gas flow,_O2 concentration,_CO2 Concentration, pump flow rate, swing bed speed and angle and other parameters, and can set the deviation alarm conditions of each parameter, if the alarm condition is triggered, the alarm information is displayed, the curve and information of each run are automatically saved in the database, and all operating processes, process data, and editing reports can be easily accessed and analyzed, and three-level user access rights can be set, and third-party data collection and monitoring systems can be communicated.