Microbial strain screening mini series -0.1 liter -1 liter micro glass fermentation tank (ex situ sterilization), truly small microbial strain screening system BLBIO mini series
0.2L Magnetic Stirring Microbioreactor BLBIO-0.2GC-mini
0.5 liter mechanically stirred micro bioreactor BLBIO-0.5GJG mini
1 liter 8-unit micro bioreactor BLBIO mini 1GJ * 8-mini
The BLBIO-mini microbioreactor saves more than 10 times the time compared to general cultivation methods, making it the best tool for large-scale bacterial strain/cell line screening and culture condition optimization research.
Main application examples:
• Discovery of secondary metabolites or new enzyme activity in heterogeneous mixed culture products; • High throughput screening research, mutation distribution research, and construction of Escherichia coli or yeast libraries Metabolic flow research and high-throughput screening of highly active mutant strains; • Comparative studies, such as isolation and screening of clinical strains; • Optimization of growth medium for cell lines or high-yield strains;
Parameter
Description
Total volume
Maximum total volume size is 2L.
Working volume
30%~70%
The ratio between diameter and height
1:2.0~2.3
Vessel specifications and quantity
Micro fermenter quipped with 4 glass vessel systems. Maximum total volume size is 2L. The material is high temperature sterilization borosilicate glass. The tank cover distributes the inoculation port, pH electrode port, DO electrode port, 4-way feed port, temperature electrode port, and sampling port. Each tank is equipped with 2 gears Rushton stirring paddle, 1 gear axial flow oblique blade paddle, annular gas distributor, and anhydrous temperature control exhaust gas condenser.
Innovative water-free temperature module
Micro fermenter can be used for 4 tanks at the same time. The innovative water-free temperature control module can independently control the temperature of each tank without external cooling water auxiliary equipment, reducing the water environment around the fermentation. Temperature control range: 10~45℃; accuracy: ±0.1℃.
Stirring control module
Mini bioreactor can control 4 tanks at the same time, equipped with servo drive motor, speed control range: 50-1000rpm. PID control can be set for manual, automatic or cascade control.
DO control module
4 high temperature sterilization DO probes, DO display control range 0.0-150.0%, accuracy: ±1.0%. Dissolved oxygen can be controlled in forward or reverse cascade through parameters/behaviors such as stirring speed, air ventilation, and feeding. Three cascade modes of parallel, series and cross can be used to achieve more precise control;
pH control module
for 4 tanks at the same time. The acid/alkali pump can realize linkage control with the feed pump to maintain the balance of the reactor matrix concentration. 4 high temperature sterilization pH probes. The control range is 2.00-12.00±0.01;
Precision feeding pump module: 16 units, 4 watson-marlow peristaltic pumps each. Each feed pump module must be operated with independent power supply, and can be freely split and combined (non-integrated) according to the different feeding processes of each tank. Precisely control the amount of feed, realize batch, feeding, continuous and perfusion culture mode ；
Gas distribution module
for 4 tanks. Equipped with a rotameter to manually control the air intake, range: 0~2L/min
Foam/Liquid Level Control:
Foam control is realized by the foam probe sensing the liquid level and automatically adding defoamer.
The system is modular in design (non-traditional integrated), which can be split and expanded freely. Each module can run independently without interfering with each other, ensuring good repeatability and parallelism, so as to achieve rapid and accurate development of process conditions exploration. The desktop (not floor-standing) compact design of the whole machine, the size does not exceed: 1.5m*0.8m*0.9m (length, width and height) to fit the tight laboratory space.
The exhaust has a water-free condenser, no external cooling water is required
Fermenter Description:
BLBIO GJ series fermentation system is a set of laboratory glass fermentation equipment with powerful functions, which can meet various purposes of fermentation, such as batch, batch fed, and even animal cell culture.
The 10inch color touch screen is used to display all measured values and control parameters, which greatly facilitates the user’s operation. 4 peristaltic pumps are installed on the front of the control box, which can replenish acid, alkali, defoamer and medium to the fermenter.
Various parameters in fermentation such as pH, DO, temperature and rotational speed can be monitored by the controller, and all data and control parameters can be transmitted to the controller or computer.
The structure is compact, and various components can be integrated on the fermentation system.
The upper-driven direct-coupling mechanical stirring ,with simple structure and stable operation.
